Circular Dichroism Spectrometer

General information

Investigation area
Spectroscopy
Techniques
Circular Dichroism Spectrometry
Manufacturer
Applied Photophysics
Fabrication year
2013
Measured quantity
CD Absorbance; UV/VIS Absorbance
Main application
Structure detection of Biomolecules, Adsorption studies
In-situ, real-time compatible
Yes
Facility
Access to this instrument is possible via the MAPEX Core Facility for Materials Analytics

Technical aspects
Accessible wavelength are 190 nm to 250 nm and 250 nm to 400 nm and further.
Quartz cuvettes with varying path length (from 0.01mm to 10 mm) for different biomolecule concentrations (10 mg/ml - 0.01 mg/ml).
Required volumes vary between 10 µl to 3000 µl.
Biomolecules need to be solvated in chlorid-free buffers like K-phosphate buffer.
In-situ capabilities
Temperature ramping from 20 °C to 95 °C. Titration for pH-depended studies.

Updated by: MAPEX